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For example, grasshoppers in the subfamilies Acridinae and Oedipodinae generally prefer monocots and dicotyledons, respectively, whereas the Catantopinae will feed on both 9. Generally, grasshopper feeding habits can be determined and broadly categorized by employing field cages 101112examining the morphological structure of grasshopper mouthparts 1314analyzing the morphology of grasshopper droppings 11151617or examining the structure Adult singles dating in xilinhot the grasshopper alimentary canal 18 Microscopic examination and DNA barcoding of gut content or faeces have also been used to determine the food preference of grasshoppers 2021222324 But these methods have certain limitations, such as long experimental period, lack of quantitative precision, and inability to accurately measure feeding in individuals, where food intake is obviously in comparatively small amounts.

Because these approaches may in some cases have little practical application to understanding grasshopper biology, techniques that are more simple and quantitative offer new ways to understand insect biology at an individual level while providing insights into the drivers of population level effects in plant communities. Of the various techniques, real-time PCR is a highly sensitive technology, with high signal-to-noise ratio and high throughput, which can be used to evaluate insect diets It requires a special thermocycler and specific reagents used for fluorescence, but does not require equipment associated with gel analysis used in conventional PCR, that could detect a higher frequency of insect food consumption than conventional PCR when both tests are run at optimal conditions 28 It has been used in pathology and vector insect research 303132to investigate the relationship between humans and mosquitoes 33as well as insect classification, insect nucleotide polymorphism, insect toxicology 34insect physiology 35and mostly focusing on insect molecular ecology 28293637383940 et al.

To date, this method has not yet been utilized to study the diet structure of grasshopper. We examined food intake and preference of two grasshoppers, Oedaleus asiaticus B. Bienko and Dasyhippus barbipes Fischer-Waldheim.

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